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论文类型：期刊论文

发表时间：2017-09-01

发表刊物：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS

收录刊物：Scopus、SCIE、PubMed

卷号：73

期号：Pt 9

页面范围：505-510

ISSN号：2053-230X

关键字：toxin-antitoxin systems; HicAB; persister cells; cell stress; Escherichia coli

摘要：Toxin-antitoxin (TA) systems are widespread in both bacteria and archaea, where they enable cells to adapt to environmental cues. TA systems play crucial roles in various cellular processes, such as programmed cell death, cell growth, persistence and virulence. Here, two distinct forms of the type II toxin-antitoxin complex HicAB were identified and characterized in Escherichia coli K-12, and both were successfully overexpressed and purified. The two proposed forms, HicAB(L) and HicAB(S), differed in the presence or absence of a seven-amino-acid segment at the N-terminus in the antitoxin HicB. The short form HicABS readily crystallized under the conditions 0.1 M Tris-HCl pH 8.0, 20%(w/v) PEG 6000, 0.2 Mammonium sulfate. The HicAB(S) crystal diffracted and data were collected to 2.5 angstrom resolution. The crystal belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 67.04, b = 66.31, c = 120.78 angstrom. Matthews coefficient calculation suggested the presence of two molecules each of HicA and HicB(S) in the asymmetric unit, with a solvent content of 55.28% and a Matthews coefficient (V-M) of 2.75 angstrom 3 Da(-1).