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Indexed by:期刊论文

Date of Publication:2016-11-01

Journal:Micromachines

Included Journals:SCIE、EI、PubMed、Scopus

Volume:7

Issue:11

ISSN No.:2072-666X

Key Words:Y-shaped microfluidic device; wall shear stress; adenosine triphosphate (ATP) signal; combined effect; vascular endothelial cells; calcium dynamics

Abstract:The intracellular calcium dynamics in vascular endothelial cells (VECs) in response to wall shear stress (WSS) and/or adenosine triphosphate (ATP) have been commonly regarded as an important factor in regulating VEC function and behavior including proliferation, migration and apoptosis. However, the effects of time-varying ATP signals have been usually neglected in the past investigations in the field of VEC mechanobiology. In order to investigate the combined effects of WSS and dynamic ATP signals on the intracellular calcium dynamic in VECs, a Y-shaped microfluidic device, which can provide the cultured cells on the bottom of its mixing micro-channel with stimuli of WSS signal alone and different combinations of WSS and ATP signals in one single micro-channel, is proposed. Both numerical simulation and experimental studies verify the feasibility of its application. Cellular experimental results also suggest that a combination of WSS and ATP signals rather than a WSS signal alone might play a more significant role in VEC Ca2+ signal transduction induced by blood flow.