Release Time:2019-11-01  Hits:

Indexed by: Journal Papers

Date of Publication: 2019-11-01

Journal: JOURNAL OF MICROMECHANICS AND MICROENGINEERING

Included Journals: SCIE

Volume: 29

Issue: 11

ISSN: 0960-1317

Key Words: intracellular calcium response; dynamic mechanical stimuli; mechanotransduction; cancer cell phenotype; microfluidic device

Abstract: To probe intracellular calcium response while single cells squeeze through narrow channels, we built a high-throughput microfluidic device where single cells can be trapped efficiently and stimulated mechanically. With this device, dozens of single cells' dynamic morphologies and intracellular [Ca2+] responses under dynamic mechanical stimuli can be monitored simultaneously. We observed a two-peak [Ca2+] response, which was closely coupled together with the dynamic cellular squeezing process. This type of [Ca2+] response, to our knowledge, was observed for the first time. We also investigated the role of the cytoskeleton in the [Ca2+] response and found that the cytoskeleton was an important regulator of [Ca2+] signaling during the cellular squeezing process. In addition, we investigated the difference between the two-peak [Ca2+] responses of Hela cells and HUVECs and found that one characteristic parameter could distinguish Hela cells from HUVECs.