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Date of Publication:2015-01-01

Journal:水动力学研究与进展A辑

Issue:6

Page Number:643-649

ISSN No.:1000-4874

Abstract:The cellular biological behavior, such as proliferation, differentiation and apoptosis, is regulated by the cellular niche. The physical and chemical factors, such as fluid shear stress and biochemical factors, are included in the cellular microenvironment. In order to investigate the cultured cells in vitro in response to the combined effects of shear stress and biochemical factors in cellular microenvironment, a Y-type microfluidic device which has an inlet A with biochemical substance and an inlet B without biochemical substance is designed based upon the principles of fluid mechanics and mass transfer. Three different types of stimuli, including dynamic shear stress alone, dynamic shear stress and dynamic biochemical factor, as well as dynamic shear stress and static biochemical factor, could be provided in the mixing channel by keeping a constant volumetric flow rate at the inlet A and dynamically changing the volumetric flow rate at the inlet B. The dynamic shear stress and the concentration profiles of biochemical factors are obtained by numerically solving the equations governing the unsteady laminar flow and Taylor-Aris dispersion. The experimental validation is also presented by using fluorescent solution. Both the results from numerical simulations and experimental studies have shown the microfluidic device designed in this paper could provide a possibility for further investigating the biological cells cultured in vitro in response to combined effects of shear stress and biochemical factors. ? 2015, Publishing House for Journal of Hydrodynamics. All right reserved.

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