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Indexed by:Journal Papers

Date of Publication:2015-10-01

Journal:BIOMECHANICS AND MODELING IN MECHANOBIOLOGY

Included Journals:PubMed、SCIE、EI、Scopus

Volume:14

Issue:5

Page Number:979-993

ISSN No.:1617-7959

Key Words:Calcium ion channels; P2X(4); Shear flow; Direct activation; Indirect activation; Dynamic modeling

Abstract:The calcium signaling plays a vital role in flow-dependent vascular endothelial cell (VEC) physiology. Variations in fluid shear stress and ATP concentration in blood vessels can activate dynamic responses of cytosolic-free through various calcium channels on the plasma membrane. In this paper, a novel dynamic model has been proposed for transient receptor potential vanilloid 4 -mediated intracellular calcium dynamics in VECs induced by fluid shear stress and ATP. Our model includes signaling pathways through P2Y receptors and channels (indirect mechanism) and captures the roles of the compound channels in VEC signaling in response to fluid shear stress (direct mechanism). In particular, it takes into account that the compound channels are regulated by intracellular and concentrations. The simulation studies have demonstrated that the dynamic responses of calcium concentration produced by the proposed model correlate well with the existing experimental observations. We also conclude from the simulation studies that endogenously released ATP may play an insignificant role in the process of intracellular response to shear stress.