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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：光电工程与仪器科学学院院长、党委副书记
其他任职：辽宁省先进光电子技术重点实验室副主任，大连市新型功能材料与光电子器件重点实验室主任，中国超材料学会理事，中国机械工程学会极端制造分委员会委员，国际先进材料学会会士，Microsystems&Nanoengineering、chemosensors等期刊副编辑
性别：男
毕业院校：布里斯托尔大学
学位：博士
所在单位：光电工程与仪器科学学院
学科：光学工程. 电磁场与微波技术. 光学. 测试计量技术及仪器. 精密仪器及机械
办公地点：研教楼706
联系方式：0411-84706156
电子邮箱：caotun1806@dlut.edu.cn
A Microfluidic Micropipette Aspiration Device to Study Single-Cell Mechanics Inspired by the Principle of Wheatstone Bridge
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论文类型：期刊论文
发表时间：2019-02-01
发表刊物：MICROMACHINES
收录刊物：PubMed、SCIE、EI
卷号：10
期号：2
ISSN号：2072-666X
关键字：micropipette aspiration; microfluidics; single-cell mechanics; Wheatstone bridge
摘要：The biomechanical properties of single cells show great potential for early disease diagnosis and effective treatments. In this study, a microfluidic device was developed for quantifying the mechanical properties of a single cell. Micropipette aspiration was integrated into a microfluidic device that mimics a classical Wheatstone bridge circuit. This technique allows us not only to effectively alter the flow direction for single-cell trapping, but also to precisely control the pressure exerted on the aspirated cells, analogous to the feature of the Wheatstone bridge that can precisely control bridge voltage and current. By combining the micropipette aspiration technique into the microfluidic device, we can effectively trap the microparticles and Hela cells as well as measure the deformability of cells. The Young's modulus of Hela cells was evaluated to be 387 +/- 77 Pa, which is consistent with previous micropipette aspiration studies. The simplicity, precision, and usability of our device show good potential for biomechanical trials in clinical diagnosis and cell biology research.