location: Current position: Home >> Scientific Research >> Paper Publications

Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis

Hits:

Indexed by:期刊论文

Date of Publication:2018-05-01

Journal:ANALYTICAL CHEMISTRY

Included Journals:PubMed、SCIE

Volume:90

Issue:9

Page Number:5825-5832

ISSN No.:0003-2700

Abstract:Despite rapid progresses in single-cell analysis technologies, efforts to control the three-dimensional micro-environment for single cell measurements have been lacking. Here, we report a simple method to incorporate three-dimensional scaffolds, including polyvinylidene fluoride (PVDF) membranes and PVDF membrane replicated analog polydimethylsiloxane, into multiplexed single cell secretomic analysis platforms (including a microwell array and a single cell barcode microchip) to mimic the extracellular physical matrix and mechanical support for single cells. Applying this platform to brain tumor cell line U87 to investigate single cell protein secretion behavior on different substrates, we revealed that single cell protein secretions were regulated differently in three-dimensional (3D) microenvironments. This finding was further verified with intracellular cytokine staining, highlighting the significance of 3D single cell biomimetic platform can be easily adaptable to other three-dimensional cell culture scaffolds or other single cell assays and may become a broadly applicable three-dimensional single cell analysis system to study the effect of microenvironment conditions on cellular functional heterogeneity in vitro.

Pre One:Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device

Next One:原代肾小管上皮细胞的提取和培养方法的优化