Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2015-04-01

Journal: ELECTROPHORESIS

Included Journals: Scopus、SCIE、PubMed

Volume: 36

Issue: 7-8

Page Number: 986-993

ISSN: 0173-0835

Key Words: Beta-agonists; Chemiluminescence; Microflow injection analysis; Microfluidic chip

Abstract: -Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the -agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the -agonists. The chip was fabricated from two glass plates (64 mmx32 mm) with microchannels of 200 m width and 100 m depth. The detection limits were achieved at 2.0x10(-8)mol/L of ractopamine, 1.0x10(-8)mol/L of terbutaline and 5.0x10(-7)mol/L of salbutamol. In this report, our method was applied for determination of the -agonists in swine hair from three different sources with satisfactory results.