Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2014-12-21

Journal: ANALYTICAL METHODS

Included Journals: Scopus、EI、SCIE

Volume: 6

Issue: 24

Page Number: 9684-9690

ISSN: 1759-9660

Abstract: beta-Agonists are a group of illegal but widely used feed additives in the stockbreeding industry. In order to achieve simple-to-use, fast and high-throughput testing of this banned chemical, herein we suggest a paper-based analytical device on which a chemiluminescence diminishment method was performed. In this approach, extracts from swine hair samples as well as luminescent reagents, such as luminol and potassium periodate solution, in a low volume were applied to our device. It was found that the light emission was diminished by the beta-agonists extracted from the swine hair samples. The degree of diminishment is proportional to the concentration of the beta-agonists from 1.0 x 10(-5) to 1.0 x 10(-8) mol L-1. Also, the concentrations of solutions for chemiluminescence were optimized. The mechanism and reaction kinetics of chemiluminescence were discussed as well. The detection limit was obtained as 1.0 x 10(-9) mol L-1, and recoveries from 96% to 110% were achieved, both of which suggested that our method will be favourable in field applications for swine hair samples.