Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2012-02-01

Journal: BIOANALYSIS

Included Journals: PubMed、SCIE

Volume: 4

Issue: 4

Page Number: 453-463

ISSN: 1757-6180

Abstract: Traditional 'macroscopic' pharmacokinetics (PK) investigates the fate of DRUGS or toxicants administered externally to living organisms, described by the extent and rate of absorption, distribution, metabolism and excretion. However, how a single cell affects a specific pharmaceutical after administration still remains a largely untouched area, primarily due to the technical restrictions imposed by minute amounts of chemicals involved. With the fast development of high-temporal and spatial-resolution detection techniques and single-cell handling techniques. becomes possible to pursue single-cell PK. This review summarizes useful methodological and experimental techniques to investigate PK at the level of the single cell, including the microfluidics-based single-cell manipulation and the MS and electrochemical methods for single-cell analysis.