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Indexed by:期刊论文

Date of Publication:2012-02-01

Journal:BIOANALYSIS

Included Journals:SCIE、PubMed

Volume:4

Issue:4

Page Number:453-463

ISSN No.:1757-6180

Abstract:Traditional 'macroscopic' pharmacokinetics (PK) investigates the fate of DRUGS or toxicants administered externally to living organisms, described by the extent and rate of absorption, distribution, metabolism and excretion. However, how a single cell affects a specific pharmaceutical after administration still remains a largely untouched area, primarily due to the technical restrictions imposed by minute amounts of chemicals involved. With the fast development of high-temporal and spatial-resolution detection techniques and single-cell handling techniques. becomes possible to pursue single-cell PK. This review summarizes useful methodological and experimental techniques to investigate PK at the level of the single cell, including the microfluidics-based single-cell manipulation and the MS and electrochemical methods for single-cell analysis.