个人信息Personal Information
副教授
博士生导师
硕士生导师
主要任职:无
性别:男
毕业院校:中科院大连化物所
学位:博士
所在单位:化工学院
学科:分析化学. 药物分析学. 生物工程与技术
办公地点:化工实验楼G-215
联系方式:yluo@dlut.edu.cn
电子邮箱:yluo@dlut.edu.cn
Live cell refractometry based on non-SPR microparticle sensor
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论文类型:期刊论文
发表时间:2013-06-01
发表刊物:ELECTROPHORESIS
收录刊物:SCIE、PubMed、Scopus
卷号:34
期号:11,SI
页面范围:1526-1529
ISSN号:0173-0835
关键字:Differential interference contrast microscopy; Living cell; Polystyrene microparticles; Refractive index
摘要:Unlike the nanoparticles with surface plasmon resonance, the optical response of polystyrene microparticles (PSMPs) is insensitive to the chemical components of the surrounding medium under the wavelength-dependent differential interference contrast microscopy. This fact is exploited for the measurement of the refractive index of cytoplasm in this study. PSMPs of 400 nm in diameter were loaded into the cell to contact cytoplasm seamlessly, and the refractive index information of cytoplasm could be extracted by differential interference contrast microscopy operated at 420 nm illumination wavelength through the contrast analysis of PSMPs images.