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论文类型：期刊论文

发表时间：2012-02-01

发表刊物：BIOANALYSIS

收录刊物：SCIE、PubMed

卷号：4

期号：4

页面范围：453-463

ISSN号：1757-6180

摘要：Traditional 'macroscopic' pharmacokinetics (PK) investigates the fate of DRUGS or toxicants administered externally to living organisms, described by the extent and rate of absorption, distribution, metabolism and excretion. However, how a single cell affects a specific pharmaceutical after administration still remains a largely untouched area, primarily due to the technical restrictions imposed by minute amounts of chemicals involved. With the fast development of high-temporal and spatial-resolution detection techniques and single-cell handling techniques. becomes possible to pursue single-cell PK. This review summarizes useful methodological and experimental techniques to investigate PK at the level of the single cell, including the microfluidics-based single-cell manipulation and the MS and electrochemical methods for single-cell analysis.