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论文类型： 期刊论文

发表时间： 2017-02-01

发表刊物： JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录刊物： SCIE、EI、PubMed

卷号： 123

期号： 2

页面范围： 141-146

ISSN号： 1389-1723

关键字： Biofuels production; Saccharomyces cerevisiae; Yeast stress tolerance; Cell flocculation; Zinc supplementation; Metabolic engineering

摘要： Budding yeast Saccharomyces cerevisiae is widely studied for the production of biofuels from lignocellulosic biomass. However, economic production is currently challenged by the repression of cell growth and compromised fermentation performance of S. cerevisiae strains in the presence of various environmental stresses, including toxic level of final products, inhibitory compounds released from the pretreatment of cellulosic feedstocks, high temperature, and so on. Therefore, it is important to improve stress tolerance of S. cerevisiae to these stressful conditions to achieve efficient and economic production. In this review, the latest advances on development of stress tolerant S. cerevisiae strains are summarized, with the emphasis on the impact of cell flocculation and zinc addition. It was found that cell flocculation affected ethanol tolerance and acetic acid tolerance of S. cerevisiae, and addition of zinc to a suitable level improved stress tolerance of yeast cells to ethanol, high temperature and acetic acid. Further studies on the underlying mechanisms by which cell flocculation and zinc status affect stress tolerance will not only enrich our knowledge on stress response and tolerance mechanisms of S. cerevisiae, but also provide novel metabolic engineering strategies to develop robust yeast strains for biofuels production. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.