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论文类型： 期刊论文

发表时间： 2016-03-01

发表刊物： FEMS MICROBIOLOGY LETTERS

收录刊物： SCIE、PubMed、Scopus

卷号： 363

期号： 5

页面范围： fnw023

ISSN号： 0378-1097

关键字： Clostridium acetobutylicum; calcium; zinc; synergistic effect; xylose; butanol tolerance

摘要： Biobutanol outperforms bioethanol as an advanced biofuel, but is not economically competitive in terms of its titer, yield and productivity associated with feedstocks and energy cost. In this work, the synergistic effect of calcium and zinc was investigated in the acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum using glucose, xylose and glucose/xylose mixtures as carbon source(s). Significant improvements associated with enhanced glucose/xylose utilization, cell growth, acids re-assimilation and butanol biosynthesis were achieved. Especially, the maximum butanol and ABE production of 16.1 and 25.9 g L-1 were achieved from 69.3 g L-1 glucose with butanol/ABE productivities of 0.40 and 0.65 g L-1 h(-1) compared to those of 11.7 and 19.4 g/L with 0.18 and 0.30 g L-1 h(-1) obtained in the control respectively without any supplement. More importantly, zinc was significantly involved in the butanol tolerance based on the improved xylose utilization under various butanol-shock conditions (2, 4, 6, 8 and 10 g L-1 butanol). Under the same conditions, calcium and zinc co-supplementation led to the best xylose utilization and butanol production. These results suggested that calcium and zinc could play synergistic roles improving ABE fermentation by C. acetobutylicum.