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Indexed by:期刊论文

Date of Publication:2015-01-26

Journal:OPTICS EXPRESS

Included Journals:EI、SCIE、Scopus

Volume:23

Issue:2

Page Number:1232-1239

ISSN No.:1094-4087

Abstract:The ability to characterize the Point Spread Function(PSF) is crucial in practical microscopy, but requires knowledge of the complex PSF for approaches that detect fields instead of intensities. Here we experimentally measure and theoretically model the volumetric amplitude and phase response of an Interferometric Cross-polarisation Microscope to demonstrate the technique's capability to provide confocal-like images of weakly birefringent structures in living cells. We find the axial FWHM of the amplitude PSF to be 0 : 70 +/- 0 : 01 mu m and 0 : 83 mu m for model and measurement, respectively, on par with confocal microscopy. Ultimately retaining both amplitude and phase information will however enable approaches for improved localisation of objects. (C) 2015 Optical Society of America