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论文类型：期刊论文

发表时间：2016-10-01

发表刊物：JOURNAL OF SEPARATION SCIENCE

收录刊物：SCIE、EI、PubMed、Scopus

卷号：39

期号：20

页面范围：3860-3867

ISSN号：1615-9306

关键字：Basic compounds; Hydrophobicity; Reversed-phase eluents; Silica; Silicon oxynitride

摘要：Unbonded silicon oxynitride and silica high-performance liquid chromatography stationary phases have been evaluated and compared for the separation of basic compounds of differing molecular weight, pKa, and log D using aqueous/organic mobile phases. The influences of percentage of organic modifier, buffer pH, and concentration in the mobile phase on base retention were investigated on unbonded silicon oxynitride and silica phases. The results confirmed that unbonded silicon oxynitride and silica phases demonstrated excellent separation performance for model basic compounds and both the unbonded phases examined possessed a hydrophobic/adsorption and ion-exchange character. The silicon oxynitride stationary phase exhibited high hydrophilicity compared with silica with a reversed-phase mobile phase. An ion-exclusion-type mechanism becomes predominant for the separation of three aimed bases on the silicon oxynitride column at pH 2.8. Different from silicon oxynitride stationary phase, no obvious change for the retention time of three model bases on silica stationary phase at pH 2.8 can be observed.