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论文类型：期刊论文

发表时间：2020-03-01

发表刊物：ANALYTICAL AND BIOANALYTICAL CHEMISTRY

收录刊物：PubMed、EI、SCIE

卷号：412

期号：7,SI

页面范围：1497-1508

ISSN号：1618-2642

关键字：Mesoporous poly-melamine-formaldehyde (mPMF); Glycopeptide enrichment; Mass spectrometry; Hydrophilic interaction liquid chromatography (HILIC); Sialylated glycopeptide

摘要：Analysis of glycoprotein sialylation is challenging due to the relatively low abundance of sialylated glycopeptides (SGPs) in complex biosamples and low signals of SGPs in mass spectrometry. In this study, a mesoporous poly-melamine-formaldehyde (mPMF) polymer was prepared and utilized as the high-efficiency sorbent for SGPs. The mPMF polymer featured high surface area (755.4 m(2) g(-1)) and high density of amine and triazine functional groups. This polymer demonstrated high enrichment selectivity (resistant to 100 molar fold interference of BSA) and superior adsorption capacity (560 mg g(-1)) for SGPs. The high performance of mPMF toward SGPs ascribes to the unique physicochemical properties of mPMF and high density of accessible binding sites for glycopeptides. Further application of mPMF to HeLa S3 cell lysate resulted in 576 characterized glycopeptides with 218 unique glycosylation sites. This finding provides a new choice of promising extraction approach for characterization of protein glycosylation.
   Graphical abstract A mesoporous poly-melamine-formaldehyde (mPMF) polymer was prepared and utilized as the high-efficiency enrichment sorbent for sialylated glycopeptides (SGPs).