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论文类型：期刊论文

发表时间：2015-04-01

发表刊物：JOURNAL OF SEPARATION SCIENCE

收录刊物：SCIE、EI、PubMed、Scopus

卷号：38

期号：8

页面范围：1271-1276

ISSN号：1615-9306

关键字：Carbohydrates; Hydrophilic interaction liquid chromatography; Silica; Silicon oxynitride; Stationary phases

摘要：The retention characteristics of a silicon oxynitride stationary phase for carbohydrate separation were studied in hydrophilic interaction chromatography mode. Four saccharides including mono-, di-, and trisaccharides were employed to investigate the effects of water content and buffer concentration in the mobile phase on hydrophilic interaction liquid chromatography retention. For the tested saccharides, the silicon oxynitride column demonstrated excellent performance in terms of separation efficiency, hydrophilicity, and interesting separation selectivity for carbohydrates compared to the bare silica stationary phase. Finally, the silicon oxynitride hydrophilic interaction liquid chromatography column was employed in the separation of complex samples of fructooligosaccharides, saponins, and steviol glycoside from natural products. The resulting chromatograms demonstrated good separation efficiency and longer retention compared with silica, which further confirmed the advantages and potential application of silicon oxynitride stationary phase for hydrophilic interaction liquid chromatography separation.