宋波

个人信息Personal Information

副教授

博士生导师

硕士生导师

性别:男

毕业院校:大连化物所

学位:博士

所在单位:化学学院

学科:分析化学

办公地点:大连理工大学西部校区化工综合楼D313

联系方式:+86-411-84986042

电子邮箱:bo.song@dlut.edu.cn

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Quantitative Monitoring and Visualizing of Hydrogen Sulfide in vivo by A Ruthenium(II) Complex-based Luminescence Probe with New Recognition Moiety.

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论文类型:期刊论文

发表时间:2018-02-02

发表刊物:Angewandte Chemie (International ed. in English)

收录刊物:PubMed

ISSN号:1521-3773

关键字:Hydrogen sulfide,analytical method,in vivo sensing,luminescent imaging,ruthenium complex

摘要:Development of novel bioanalytical methods for monitoring of H2S is the key to understand the physiological and pathological functions of this gasotransmitter in live organisms. Herein, a ruthenium(II) complex-based luminescence probe, Ru-MDB, was developed by introducing a new H2S responsive masking moiety to red emitting RuII luminophore. Cleavage of this masking group by a H2S-triggered reaction leads to the luminescence "OFF-ON" response. The long-lived emissions of Ru-MDB and its reaction product with H2S allowed quantitative detection of H2S in autofluorescence-rich human sera and adult zebrafish organs with time-gated luminescence mode. Ru-MDB showed red emission, large Stokes shift, high specificity and sensitivity for H2S detection, and low cytotoxicity, which enabled the imaging and flow cytometry analysis of lysosomal H2S generation in inflammation of live cells under drug stimulation. Monitoring of H2S in live Daphnia magna, zebrafish embryos, adult zebrafish, and mice was then conducted by in vivo imaging using Ru-MDB as a probe. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.