个人信息Personal Information
副教授
博士生导师
硕士生导师
性别:男
毕业院校:大连化物所
学位:博士
所在单位:化学学院
学科:分析化学
办公地点:大连理工大学西部校区化工综合楼D313
联系方式:+86-411-84986042
电子邮箱:bo.song@dlut.edu.cn
Homogeneous time-resolved fluoroimmunoassay of microcystin-LR using layered WS2 nanosheets as a transducer
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论文类型:期刊论文
发表时间:2017-06-01
发表刊物:METHODS AND APPLICATIONS IN FLUORESCENCE
收录刊物:SCIE、PubMed
卷号:5
期号:2
页面范围:024007
ISSN号:2050-6120
关键字:time-resolved fluoroimmunoassay; microcystin-LR; WS2 nanosheets; luminescent Eu3+ complex; immunosensing
摘要:Ahomogeneous time-resolved fluoroimmunoassay method for rapid and sensitive detection of microcystin-LR (MC-LR) in water samples was developed based on the interaction between water-soluble WS2 nanosheets and the conjugate of MC-LR with a luminescent Eu3+ complex BHHBCB-Eu3+ (BHHBCB: 1,2-bis[4 '-(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)-benzyl]-4-chlorosulfobenzene). The large lateral dimensions and high surface areas of two-dimensional layered WS2 nanosheets enable easy adsorption of the MC-LR-BHHBCB-Eu3+ conjugate, that lead to efficient quenching of the luminescence of Eu3+ complex via energy transfer or electron transfer process. However, the addition of monoclonal anti-MC-LR antibody can induce the formation of MC-LRBHHBCB- Eu3+/antibody immune complex, which prevents the interaction between WS2 nanosheets and MC-LR-BHHBCB-Eu3+ to result in the restoration of Eu3+ luminescence. This signal transduction mechanism made it possible for analysis of the target MC-LR in a homogeneous system. The present method has advantages of rapidity and simplicity since the B/F (bound reagent/free reagent) separation steps, the solid-phase carrier and antibody labeling or modification process are not necessary. The proposed immunosensing system displayed a wide linear range, good precision and accuracy, and comparable sensitivity with a detection limit of 0.3 mu gl(-1), which satisfied the World Health Organization (WHO) provisional guideline limit of 1.0 mu gl(-1) for MC-LR in drinking water.