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论文类型：期刊论文

发表时间：2017-04-21

发表刊物：JOURNAL OF MATERIALS CHEMISTRY B

收录刊物：SCIE、EI

卷号：5

期号：15

页面范围：2849-2855

ISSN号：2050-750X

摘要：Bioresponsive luminescence probes based on lanthanide complexes have shown great utility in a variety of time-gated luminescence bioassays. However, functional lanthanide complexes that can target individual organelles for probing biospecies therein have rarely been investigated. In this work, a unique Eu3+ complex, Mito-NPSTTA-Eu3+, was designed and synthesized as a probe for the time-gated luminescence sensing of HOCl inside the mitochondria of living cells. The probe showed a fast, highly sensitive and selective luminescence response to HOCl with a wide available pH range (pH 3-10), and highly specific mitochondria-localization performance. Taking advantage of time-gated luminescence bioimaging and the excellent properties of the Eu3+ complex, the generation of endogenous HOCl in RAW 264.7 cells and the uptake of exogenous HOCl by zebrafish were successfully imaged, respectively. The results demonstrated the feasibility of Mito-NPSTTA-Eu3+ for the imaging of mitochondrial HOCl, and validated the potential of our strategy for the design of lanthanide complex-based organelle-targeting bioresponsive probes.