宋波

个人信息Personal Information

副教授

博士生导师

硕士生导师

性别:男

毕业院校:大连化物所

学位:博士

所在单位:化学学院

学科:分析化学

办公地点:大连理工大学西部校区化工综合楼D313

联系方式:+86-411-84986042

电子邮箱:bo.song@dlut.edu.cn

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Enabling the Triplet of Tetraphenylethene to Sensitize the Excited State of Europium(III) for Protein Detection and Time-Resolved Luminescence Imaging

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论文类型:期刊论文

发表时间:2016-12-01

发表刊物:ADVANCED SCIENCE

收录刊物:SCIE、EI、PubMed、Scopus

卷号:3

期号:12

页面范围:1600146

ISSN号:2198-3844

关键字:europium complex,luminescence probe,protein detection,time‐resolved imaging

摘要:A tetraphenylethene (TPE) group that exhibits aggregation-induced emission is incorporated into the ligand of a Eu(III) complex (TPEEu) to sensitize the excited state of Eu(III). In steady-state measurements, TPEEu exhibits weak luminescence when dissolved in aqueous solutions even at a high concentration level, but emits strong fluorescence of TPE and phosphorescence of Eu(III) upon binding with bovine serum albumin. With a delay time of 0.05 ms and a gate time of 1.0 ms in time-resolved measurements, only phosphorescent emission of Eu(III) is observed with a high on/off ratio. Moreover, this probe is successfully used in time-resolved luminescence imaging to eliminate the background signal from biological autofluorescence without a washing process. This work provides a general strategy in designing Ln(III) complexes for detecting a broad range of biological molecules.