个人信息Personal Information
副教授
博士生导师
硕士生导师
性别:男
毕业院校:大连化物所
学位:博士
所在单位:化学学院
学科:分析化学
办公地点:大连理工大学西部校区化工综合楼D313
联系方式:+86-411-84986042
电子邮箱:bo.song@dlut.edu.cn
Iridium(III) Complex-Based Activatable Probe for Phosphorescent/Time-Gated Luminescent Sensing and Imaging of Cysteine in Mitochondria of Live Cells and Animals
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论文类型:期刊论文
发表时间:2019-01-28
发表刊物:CHEMISTRY-A EUROPEAN JOURNAL
收录刊物:SCIE、PubMed、Scopus
卷号:25
期号:6
页面范围:1498-1506
ISSN号:0947-6539
关键字:cysteine; iridium; phosphorescence; sensing and imaging; time-gated luminescence
摘要:This study reports an activatable iridium(III) complex probe for phosphorescence/time-gated luminescence detection of cysteine (Cys) in vitro and in vivo. The probe, [Ir(ppy)(2)(NTY-bpy)](PF6) [ppy: 2-phenylpyridine; NTY-bpy: 4-methyl-4 '-(2-nitrovinyl)-2,2 '-bipyridine], is developed by incorporating a strong electron-withdrawing group, nitroolefin, into a bipyridine ligand of the Ir-III complex. The luminescence of the probe is quenched owing to the intramolecular charge transfer (ICT) process, but switched on by a specific recognition reaction between the probe and Cys. [Ir(ppy)(2)(NTY-bpy)](PF6) shows high sensitivity and selectivity for Cys detection and good biocompatibility. The long-lived emission of [Ir(ppy)(2)(NTY-bpy)](PF6) allows time-gated luminescence analysis of Cys in cells and human sera. These properties make it convenient for the phosphorescence and time-gated luminescence imaging and flow cytometry analysis of Cys in live samples. The Cys images in cancer cells and inflamed macrophage cells reveal that [Ir(ppy)(2)(NTY-bpy)](PF6) is distributed in mitochondria after cellular internalization. Visualizations and flow cytometry analysis of mitochondrial Cys levels and Cys-mediated redox activities of live cells are achieved. By using [Ir(ppy)(2)(NTY-bpy)](PF6) as a probe, in vivo sensing and imaging of Cys in D. magna, zebrafish, and mice are then demonstrated.