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论文类型：期刊论文

发表时间：2014-09-01

发表刊物：JOURNAL OF FLUORESCENCE

收录刊物：SCIE、EI、PubMed、Scopus

卷号：24

期号：5

页面范围：1537-1544

ISSN号：1053-0509

关键字：Terbium complex; Luminescent probe; Zinc ions; Time-resolved luminescence sensing

摘要：Luminescent probes/chemosensors based on lanthanide complexes have shown great potentials in various bioassays due to their unique long-lived luminescence property for eliminating short-lived autofluorescence with time-resolved detection mode. In this work, we designed and synthesized a new dual-chelating ligand {4'-[N,N-bis(2-picolyl)amino]methylene-2,2':6',2'-terpyridine-6,6'-diyl} bis(methylenenitrilo) tetrakis(acetic acid) (BPTTA), and investigated the performance of its Tb3+ complex (BPTTA-Tb3+) for the time-resolved luminescence sensing of Zn2+ ions in aqueous media. Weakly luminescent BPTTA-Tb3+ can rapidly react with Zn2+ ions to display remarkable luminescence enhancement with high sensitivity and selectivity, and such luminescence response can be realized repeatedly. Laudably, the dose-dependent luminescence enhancement shows a good linear response to the concentration of Zn2+ ions with a detection limit of 4.1 nM. To examine the utility of the new probe for detecting intracellular Zn2+ ions, the performance of BPTTA-Tb3+ in the time-resolved luminescence imaging of Zn2+ ions in living HeLa cells was investigated. The results demonstrated the applicability of BPTTA-Tb3+ as a probe for the time-resolved luminescence sensing of intracellular Zn2+ ions.