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Date of Publication:2022-10-06

Journal:纳米技术与精密工程

Issue:4

Page Number:246-250

ISSN No.:1672-6030

Abstract:Regarding the instability of the cell microenvironment in vitro, a chip with a stable environ-ment was designed and fabricated successfully for cell culture using microfluidics technology. The cell culture chamber was quadrilateral, which was located in the main channel of the chip. A micropillar ar-ray surrounded the chamber, and there were gaps between the micropillars and the size of gaps was less than that of the cell. The gaps around the cell chamber formed multiple transport channels. And the mi-crofluid flew in and out of the chamber simultaneously in way of convection and diffusion through the multi-channels between the micropillars, which could timely supply nutrients and remove waste. Simula-tions of velocity and pressure profile in the chambers were done for the designed structure and traditional single channel structure using COMSOL Multiphysics. And the particle image velocimetry method was used to measure the average velocity in the chamber. Results show that the velocity of microfluid in the chamber is uniform and stable, and the chip can effectively resist external disturbances.

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