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Indexed by:期刊论文

Date of Publication:2022-06-30

Journal:激光与光电子学进展

Volume:54

Issue:8

Page Number:080402

ISSN No.:1006-4125

Abstract:Microfluidic systems based on laser induced fluorescence(LIF)detection
   technology have been widely used in the field of biochemistry
   detection.Aiming at the problem of weak fluorescence intensity induced
   by a few detection samples in the microfluidic system,a microfluidic
   chip integrating a microlens array(MLA)is designed and fabricated to
   improve the intensity of the detected fluorescence.An 8*8photoresist MLA
   mold with the diameter variation coefficient of 0.36%is obtained by the
   hot melt technology.Then,a cover plate integrating a
   polydimethylsiloxane MLA is produced by the soft-lithography
   technology,and the focal length uniformity error is 7%.Afterwards,a
   micro-channel substrate is made.The substrate and the cover plate are
   packaged by the oxygen plasma bonding technology.Finally,the
   fluorescence intensity of the chip is detected by a fluorescence
   microscope, and the chip is injected by fluorescein isothiocyanate
   fluorescent dye solution with concentration of 10mumol·L~(-1). The
   result shows that the fluorescence intensity with the microlens is
   improved about 2.2times as much as the fluorescence intensity without
   the microlens.

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