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论文类型： 期刊论文

发表时间： 2018-01-17

发表刊物： ACS APPLIED MATERIALS & INTERFACES

收录刊物： SCIE、EI、PubMed

卷号： 10

期号： 2

页面范围： 1499-1507

ISSN号： 1944-8244

关键字： Pim-1 kinase; biomarkei-targeting; cancer cell imaging mitochondria localization; in vivo imaging

摘要： Based on the fact that enzyme-targeting probes are highly sensitive and selective, a novel red-emitting probe (NB-BF) for Pim-1 kinase including three parts, fluorophore (NB), linker, and inhibitor (BF), has been designed for cancer optical imaging. In its free state, NB BF is folded and the fluorescence quenched by PET between fluorophore and inhibitor both in PBS buffer and in normal cells. Significantly, it emitted strong red fluorescence in Pim-1 overexpressed cancer cells. The specificity of NB-BF for Pim-1 kinase was directly demonstrated by gene silencing analysis. Furthermore, it is the first time to know where Pim-1 kinase mainly distributes at mitochondria with Pearson's correlation factor (R-r) of 0.965 and to provide a fluorescent tool to verify the function of the Pim-1 kinase. More importantly, NB-BF was applied in tissue imaging and preferentially labeled tumors in vivo.