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发布时间：2019-03-11

论文类型：期刊论文

发表时间：2018-01-01

发表刊物：ACS sensors

收录刊物：EI、SCIE、PubMed

卷号：3

期号：3

页面范围：735-741

ISSN号：2379-3694

关键字：superoxide anion; mitochondria-accessing; ratiometric bioimaging; fluorescent probe; fast response; inflammatory Daphnia magna

摘要：Superoxide anion (O2-), as the precursor of other reactive oxygen species (ROS), is significantly important in the maintenance of redox homeostasis and various cellular signaling pathways. Here we present a ratiometric mitochondria-accessing fluorescent probe (NA-T) based on nucleophilic substitution mechanism for real-time measuring O2-. By regulating the intramolecular charge of 1,8-naphthalimide, a ratiometric response model was obtained, which evinced 18-fold enhancement of fluorescence ratio ( I540nm/ I475nm) in the presence of O2- over other ROS with rapid response (132 s), high sensitivity (DL = 0.370 muM) and selectivity. Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane for visualizing endogenous O2- changes in mitochondria of living cells and in inflammatory Daphnia magna, indicating NA-T a potential tool for the diagnosis and research of corresponding diseases.