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论文类型： 期刊论文

发表时间： 2018-01-01

发表刊物： ACS sensors

收录刊物： PubMed、SCIE、EI

卷号： 3

期号： 3

页面范围： 735-741

ISSN号： 2379-3694

关键字： superoxide anion; mitochondria-accessing; ratiometric bioimaging; fluorescent probe; fast response; inflammatory Daphnia magna

摘要： Superoxide anion (O2-), as the precursor of other reactive oxygen species (ROS), is significantly important in the maintenance of redox homeostasis and various cellular signaling pathways. Here we present a ratiometric mitochondria-accessing fluorescent probe (NA-T) based on nucleophilic substitution mechanism for real-time measuring O2-. By regulating the intramolecular charge of 1,8-naphthalimide, a ratiometric response model was obtained, which evinced 18-fold enhancement of fluorescence ratio ( I540nm/ I475nm) in the presence of O2- over other ROS with rapid response (132 s), high sensitivity (DL = 0.370 muM) and selectivity. Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane for visualizing endogenous O2- changes in mitochondria of living cells and in inflammatory Daphnia magna, indicating NA-T a potential tool for the diagnosis and research of corresponding diseases.