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发布时间：2019-03-11

论文类型：期刊论文

发表时间：2018-08-15

发表刊物：SENSORS AND ACTUATORS B-CHEMICAL

收录刊物：EI、SCIE

卷号：267

页面范围：104-110

ISSN号：0925-4005

关键字：Fluorescent probe; Ratiometric imaging; Confined-space mechanism; Sulfite determination; FRET fabrication

摘要：A ratiometric fluorescent probe for HSO3- was assembled using a pair of Forster resonance energy transfer (FRET)-matchable fluorescent dyes within a single human serum albumin (HSA). In this work, the micro environment-sensitive dyes B1 and HE were designed and synthesized, wherein B1 located in site II as the FRET donor while HE located in site I as the FRET acceptor and receptor for HSO3-. This B1-HE FRET system exhibited a good efficiency in the sensitive, selective, and ratiometric recognition of HSO3- with a detection limit of 99 nM in aqueous media. Furthermore, this B1-HE system could realize the ratiometric imaging of HSO3- in human breast cancer cells. (C) 2018 Elsevier B.V. All rights reserved.