Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase.
Release Time:2019-03-11 Hits:
Indexed by: Journal Article
Date of Publication: 2018-05-01
Journal: Enzyme and microbial technology
Included Journals: SCIE
Volume: 112
Page Number: 1-5
ISSN: 1879-0909
Key Words: Myo-inositol; Polyphosphate glucokinase; Inositol-1-phosphate synthase; Inositol monophosphatase; Trienzymatic cascade
Abstract: Myo-inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. Here, we report a novel pathway to produce inositol from glucose by a trienzymatic cascade system involving polyphosphate glucokinase (PPGK), inositol 1-phosphate synthase (IPS) and inositol monophosphatase (IMP). The system contained three highly active enzymes, AspPPGK from Arthrobacter sp. OY3WO11, TbIPS from Trypanosoma brucei TREU927, and EcIMP from Escherichia coli. A trienzymatic cascade reaction was implemented, and the conversion ratio from glucose to inositol reached 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation. Copyright © 2018 Elsevier Inc. All rights reserved.