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Indexed by:期刊论文

Date of Publication:2018-05-01

Journal:Enzyme and microbial technology

Included Journals:SCIE

Volume:112

Page Number:1-5

ISSN No.:1879-0909

Key Words:Myo-inositol; Polyphosphate glucokinase; Inositol-1-phosphate synthase; Inositol monophosphatase; Trienzymatic cascade

Abstract:Myo-inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. Here, we report a novel pathway to produce inositol from glucose by a trienzymatic cascade system involving polyphosphate glucokinase (PPGK), inositol 1-phosphate synthase (IPS) and inositol monophosphatase (IMP). The system contained three highly active enzymes, AspPPGK from Arthrobacter sp. OY3WO11, TbIPS from Trypanosoma brucei TREU927, and EcIMP from Escherichia coli. A trienzymatic cascade reaction was implemented, and the conversion ratio from glucose to inositol reached 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation. Copyright © 2018 Elsevier Inc. All rights reserved.