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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：男
毕业院校：日本京都大学
学位：博士
所在单位：化工海洋与生命学院
电子邮箱：jnzhang@dlut.edu.cn
Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase.
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论文类型：期刊论文
发表时间：2018-05-01
发表刊物：Enzyme and microbial technology
收录刊物：SCIE
卷号：112
页面范围：1-5
ISSN号：1879-0909
关键字：Myo-inositol; Polyphosphate glucokinase; Inositol-1-phosphate synthase; Inositol monophosphatase; Trienzymatic cascade
摘要：Myo-inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. Here, we report a novel pathway to produce inositol from glucose by a trienzymatic cascade system involving polyphosphate glucokinase (PPGK), inositol 1-phosphate synthase (IPS) and inositol monophosphatase (IMP). The system contained three highly active enzymes, AspPPGK from Arthrobacter sp. OY3WO11, TbIPS from Trypanosoma brucei TREU927, and EcIMP from Escherichia coli. A trienzymatic cascade reaction was implemented, and the conversion ratio from glucose to inositol reached 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation. Copyright © 2018 Elsevier Inc. All rights reserved.