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Indexed by:期刊论文

Date of Publication:2018-01-01

Journal:INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY

Included Journals:SCIE

Volume:20

Issue:11

Page Number:2575-2578

ISSN No.:1560-8530

Key Words:Transgenic lily; Agrobacterium-mediated system; Dual anti-virus; RNA interference

Abstract:The pH7GWIWG2 (II) expression vectors of capsid protein gene sequences constructed in our lab contain Lily symptomless virus (LSV) and Lily mottle virus (LMoV). Lilium orientalus 'Sorbonne' and Lilium longiflorum,'White Heavenan' are the receptor, using Agrobacterium-mediated transformation to lily. The results show that: 'Sorbonne' and 'White Heaven' were infected with Agrobacterium bacteria (OD600 = 0.8) 15 min, fostered dark for 4 d, respectively adapter red off bacteria with cefotaxime (300 mg/L) train the filter to the selection medium containing 23.0 mg/L and 25.0 mg/L hygromycin to obtain transgenic lily. RT-PCR assay prove that the two encoding viral capsid protein gene have expressed in the lily genome, ELISA and Western blot show that the transformation of lily significantly enhance the resistance to LSV and LMoV. (C) 2018 Friends Science Publishers