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论文类型：期刊论文

发表时间：2016-05-01

发表刊物：CURRENT TOPICS IN NUTRACEUTICAL RESEARCH

收录刊物：SCIE

卷号：14

期号：2

页面范围：127-132

ISSN号：1540-7535

关键字：Apoptosis; MPP+; Parkinson's disease; Sulfated pachymaran

摘要：PC12 cells treated with 0.5 mM/MPP+ for 24 h were used as PD model in vitro to evaluate the neuroprotective effects of sulfated pachymaran (SP). Cell viability and apoptosis were detected by MTT assay, inverted microscope and Hoechst 33342 staining, respectively. Expression levels of Bcl-2, Bax and caspase-3 were detected by Western blotting. SP at the concentrations from 0.5 to 4 mg/mL is safe to PC12 cells. MPP+ treatment significantly decreased the cell viability, the expressions of Bcl-2 and Caspase-3 (P<0.01) and increased the expression of Bax (P<0.05). The pretreatment of 2 mg/mL SP significantly contributed to the restoration of cell viability (P<0.05), the up regulation of Bcl-2 (P<0.01) and Caspase-3 (P<0.01), and it also inhibited the overexpression of Bax (P<0.05). SP may inhibit apoptosis of MPP+-induced PC12 cells through the mitochondria-dependent Caspase pathway.