Release Time:2021-07-28  Hits:

Indexed by: Journal Papers

Date of Publication: 2021-01-10

Journal: CHINESE CHEMICAL LETTERS

Volume: 31

Issue: 5

Page Number: 1091-1094

ISSN: 1001-8417

Key Words: pH probe; Inflammation; Lysosome; FRET; Macrophages

Abstract: Inflammation, as the pathophysiological response of body to harmful stimuli, leads to changes in cellular microenvironment. To research pH changes in lysosomes of macrophages during inflammation, we designed a FRET (Forster resonance energy transfer) based probe, BDP-RhB. The probe showed good lysosome targeting ability, wide response range of pH from 8.0 to 4.0 with significant ratio (I-582/I-518) change from 0.6 to 3.4, and good reversibility and sustainability. By applying BDP-RhB, we found a decrease of lysosomal pH of macrophages during inflammation. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.