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Indexed by:期刊论文

Date of Publication:2021-01-10

Journal:ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Volume:59

Issue:45

Page Number:20215-20223

ISSN No.:1433-7851

Key Words:Gibbs free energy; rhodamines; single-molecule localization microscopy; spontaneously blinking dyes; super-resolution imaging

Abstract:Herein, we reported a simple, fast, and quantitative theoretical descriptor Delta G(C-O)that allows accurate predictions of a wide range of spontaneously blinking rhodamines. Delta G(C-O)denotes the Gibbs free energy differences between the closed and open forms of rhodamines and has a good linear relationship with experimental pK(cycl)values. This correlation affords an effective guide for the quantitative designs of spontaneously blinking rhodamines and eliminates trial-and-error. We have validated the predictive power of Delta G(C-O)via the development of two spontaneously blinking rhodamines of different colors and enhanced brightness. We also demonstrated their super-resolution imaging utilities in dynamic live-cell imaging. We expect that Delta G(C-O)will greatly facilitate the efficient creations of spontaneously blinking fluorophores and aid the advancements of super-resolution bioimaging techniques.