Release Time:2022-03-07  Hits:

Indexed by: Journal Article

Date of Publication: 2021-01-10

Journal: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Volume: 59

Issue: 45

Page Number: 20215-20223

ISSN: 1433-7851

Key Words: Gibbs free energy; rhodamines; single-molecule localization microscopy; spontaneously blinking dyes; super-resolution imaging

Abstract: Herein, we reported a simple, fast, and quantitative theoretical descriptor Delta G(C-O)that allows accurate predictions of a wide range of spontaneously blinking rhodamines. Delta G(C-O)denotes the Gibbs free energy differences between the closed and open forms of rhodamines and has a good linear relationship with experimental pK(cycl)values. This correlation affords an effective guide for the quantitative designs of spontaneously blinking rhodamines and eliminates trial-and-error. We have validated the predictive power of Delta G(C-O)via the development of two spontaneously blinking rhodamines of different colors and enhanced brightness. We also demonstrated their super-resolution imaging utilities in dynamic live-cell imaging. We expect that Delta G(C-O)will greatly facilitate the efficient creations of spontaneously blinking fluorophores and aid the advancements of super-resolution bioimaging techniques.