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个人信息Personal Information
副教授
硕士生导师
任职 : 昆虫反应器有机固废资源化课题组组长、 盘锦研究院_营养与微生态研究室主任、环境科学/工程/营养微生态独立PI
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：化工海洋与生命学院
学科：环境工程. 微生物学. 生物化学与分子生物学. 生物工程. 生物化工. 生态学
办公地点：大连理工大学盘锦校区
化工海洋与生命学院D05-307(办公室)
化工海洋与生命学院D05-308(实验室)
联系方式：mobile：189 0986 4927
电子邮箱：Weiping.Xu@dlut.edu.cn
Determination of estrogens by solid-phase quadruplex stable isotope dansylation coupled with liquid chromatography-high resolution mass spectrometry in environmental samples
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论文类型：期刊论文
发表时间：2020-11-01
发表刊物：TALANTA
收录刊物：SCIE
卷号：219
ISSN号：0039-9140
关键字：Multiplex isotope labeling; Solid phase extraction; LC-MS; Solid-phase labeling; Quantitative analysis
摘要：Estrogens distribute widely in the environment as endocrine-disrupting chemicals (EDCs), which have to be monitored to evaluate their environmental impact. Aim to improve the analytical throughput of liquid chromatography-high resolution mass spectrometry (LC-HRMS), a quadruplex stable isotope dansylation method was developed, with which three real samples could be quantitatively analyzed in one injection. As the estrogens were at trace level in complex matrices, magnetic solid-phase extraction (MSPE) was applied to enrich these analytes and remove the interfaces. By integrating MSPE and quadruplex stable isotope dansylation, a solid -phase quadruplex labeling method was developed for the LC-HRMS analysis of estrogen analogues. For the tested seven estrogens, the developed method showed low detection limits (0.1-0.5 ng/L for pond water and 0.01-0.05 mu g/kg for poultry manure), good precision (RSD < 5.5%) and accuracy (96.8-108.3%). The method was applied in the determination of estrogens in environmental samples, and the results revealed that all the tested estrogens were present in the estuary water (unquantifiable to 71.2 ng/L) and chicken manure (undetectable to 25.43 mu g/kg).