Title of Paper:Graphene-DNAzyme-based fluorescent biosensor for Escherichia coli detection

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Date of Publication:2018-09-01

Journal:MRS COMMUNICATIONS

Included Journals:SCIE

Volume:8

Issue:3

Page Number:687-694

ISSN No.:2159-6859

Abstract:Herein we describe the use of a new DNAzyme/graphene hybrid material as a biointerfaced sensing platform for optical detection of pathogenic bacteria. The hybrid consists of a colloidal graphene nanomaterial and an Escherichia coli-activated RNA-cleaving DNAzyme and is prepared via non-covalent self-assembly of the DNAzyme onto the graphene surface. Exposure of the hybrid material to E. coli-containing samples results in the release of the DNAzyme, followed by the cleavage-mediated production of a fluorescent signal. Given that specific RNA-cleaving DNAzymes can be created for diverse bacterial pathogens. direct interfacing of graphene materials with such DNAzymes represents a general and attractive approach for real-time, sensitive, and highly selective detection of pathogenic bacteria.