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论文类型：期刊论文

发表时间：2019-11-01

发表刊物：BIOINTERPHASES

收录刊物：PubMed、SCIE

卷号：14

期号：6

页面范围：061003

ISSN号：1934-8630

摘要：The reliability and stability of array slides are a big concern for array vendors and end users. Herein, we report on a new type of array slide with high reactivity toward DNA probes and low side reaction. A one-step surface reaction via the Michael addition involved in preparing array slides was developed and characterized by x-ray photoelectron spectroscopy, contact angle, and fluorescence labeling. The effects of array fabrication and storage conditions, i.e., spotting solution pH, high humidity, and long-term storage on the reactivity of the slides were examined. The fabricated DNA arrays could realize good hybridization efficiency (38.2% for slides with 0.88 pmol/cm(2)), low limit of detection (4 x 10(-14)M), as well as high mismatch selectivity.