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论文类型：期刊论文

发表时间：2022-06-29

发表刊物：Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities

卷号：35

期号：5

页面范围：849-859

ISSN号：1003-9015

摘要：Antibody functionalized nanoparticles (Ab-NPs) are applied in various fields due to high affinity and specificity. However, lack of control of the antibody molecular states and non-specific adsorption reduce antigen binding avidity. Herein, a series of silica nanoparticles with different surface electrical properties and charge densities were prepared. Anti α-HCG (human chorionic gonadotropin) antibody was immobilized on the surface via either physical adsorption or covalent coupling. The results indicate that the Fab domain of antibody tends outward on positively charged nanoparticles, while Fc domain tends outward with the increase of negatively charged density. Compared with covalent coupling, the immobilized antibodies via physical adsorption were reoriented because of non-specific adsorption. The tuning of antibody orientation by this method facilitates the development of bio-detection systems. © 2021, Editorial Board of "Journal of Chemical Engineering of Chinese Universities". All right reserved.

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