王世盛

个人信息Personal Information

副教授

硕士生导师

性别:男

毕业院校:中科院大连化学物理研究所

学位:博士

所在单位:化工学院

学科:药物化学. 药物工程

办公地点:大连理工大学西部校区

联系方式:Office telephone:84986200

电子邮箱:wangss@dlut.edu.cn

扫描关注

论文成果

当前位置: 中文主页 >> 科学研究 >> 论文成果

Oleanolic acid derivative SZC014 inhibit cell proliferation and induce apoptosis of human breast cancer cells in a ROS-dependent way

点击次数:

论文类型:期刊论文

发表时间:2017-01-01

发表刊物:NEOPLASMA

收录刊物:SCIE

卷号:64

期号:5

页面范围:681-692

ISSN号:0028-2685

关键字:SZC014; oleanolic acid derivative; apoptosis; breast cancer cells

摘要:Oleanolic acid (OA) and its derivatives are a novel emerging class of compounds. Although OA exhibits potent anticancer and anti-inflammatory function, the potential effect of its new derivatives (SZCO14) in human breast cancers has not been understood yet. In this investigation, we demonstrated the anticancer effect of SZCO14, a novel OA derivative and identified the possible mechanisms by which SZCO14 induced MCF-7 cell death. The biological functions of SZCO14 were validated by MTT, migration and colony formation assays in breast cancer cells. Cell apoptosis was monitored by Annexin V-FITC assay. Intracellular ROS and cell cycle were measured by flow cytometric analysis. Western blot was used to detect protein expression level. Our present results fully demonstrated that SZCO14 inhibits breast cancer cells proliferation, colony formation, and cell migration. Further investigation verified that ROS generation, apoptosis induction and Go/G, phase arrest was observed in SZCO14-treated MCF-7 cells. However, pretreatment with N-acetyl- L-cysteine (NAC), a ROS scavenger, increased the expression of procaspase-3. Additionally, SZCO14 treatment suppressed the levels of Akt, phosphorylated-Akt (p-Akt), COX-2, p-p65 in the cytoplasmic and p65 in nuclear. Furthermore, the inhibition of p65 nuclear translocation was confirmed by immunofluorescence staining. These data show that SZCO14 is an effectively selective anticancer agent against breast cancer cells, highlighting the potential use of this derivative as a breast cancer therapeutic agent.