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个人信息Personal Information
教授
博士生导师
硕士生导师
性别:女
毕业院校:大连理工大学
学位:博士
所在单位:环境学院
学科:环境工程. 环境科学
办公地点:环境学院 B317
联系方式:0411-84706913
电子邮箱:lixuehua@dlut.edu.cn
论文成果
当前位置: 大连理工大学 李雪花 >> 科学研究 >> 论文成果Determination and Prediction of the Binding Interaction between Organophosphate Flame Retardants and p53
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论文类型:期刊论文
发表时间:2014-11-01
发表刊物:CHEMICAL RESEARCH IN TOXICOLOGY
收录刊物:SCIE、Scopus
卷号:27
期号:11
页面范围:1918-1925
ISSN号:0893-228X
摘要:Organophosphate flame retardants (OF'FRs) have caused widespread concern because of the harm to the environment. In this study, to better explain the mechanism the,binding of OPFRs with the tumor suppressor gene p53, and integrated experimental -and in silico approach was used. The binding constants of 10 OPFRs were measured by surface plasmon resonance technology (SPR). The effect of OPFlts on p53 gene and protein expression in ZF4 cells was determined by quantitative real-time cR and Western blotting. Molecular docking and dynamics simulation.were explored to find that the H-bonds and hydrophobic interactions observed interactions, proper structural descriptors were used to build the nantitative structure activity relationship (QSAR) model. The current QSAR Model proyided, robustness, predictive ability, and mechanism interpretability. The pp a domain e QSAR was discussed by the Williams plot. The results showed bondarid electrostatic interaction governed the binding affinities between OPFRs and p53.