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Indexed by:期刊论文

Date of Publication:2011-07-25

Journal:NEURAL REGENERATION RESEARCH

Included Journals:Scopus、SCIE、CSCD

Volume:6

Issue:16

Page Number:1238-1242

ISSN No.:1673-5374

Key Words:magnetic resonance imaging; glioma; gene therapy; cytosine deaminase gene; bone marrow mesenchymal stem cells; lentivirus

Abstract:Bone marrow mesenchymal stem cells were isolated from C57BL mice, transfected with the cytosine deaminase (CD) gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes. C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells. C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma. Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days. MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma, as well as encased and reduced the volume of the glioma. CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.