Hits:

Indexed by:期刊论文

Date of Publication:2008-12-01

Journal:JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Included Journals:SCIE、EI、PubMed、Scopus

Volume:106

Issue:6

Page Number:610-613

ISSN No.:1389-1723

Key Words:corneal endothelial cells; vitrification; cryoprotectant; cell monolayer

Abstract:An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.