Release Time:2019-03-10  Hits:

Indexed by: Journal Article

Date of Publication: 2008-12-01

Journal: JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Included Journals: Scopus、PubMed、EI、SCIE

Volume: 106

Issue: 6

Page Number: 610-613

ISSN: 1389-1723

Key Words: corneal endothelial cells; vitrification; cryoprotectant; cell monolayer

Abstract: An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.