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论文类型：期刊论文

发表时间：2014-11-01

发表刊物：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录刊物：SCIE、EI、PubMed、Scopus

卷号：174

期号：6

页面范围：2114-2130

ISSN号：0273-2289

关键字：Neural stem cells (NSCs); In vitro culture; Collagen; Mathematical model

摘要：The aim of this study is to analyze the growth and substance metabolism of neural stem cells (NSCs) cultured in biological collagen-based scaffolds. Mass transfer and metabolism model of glucose, lactic acid, and dissolved oxygen (DO) were established and solved on MATLAB platform to obtain the concentration distributions of DO, glucose, and lactic acid in culture system, respectively. Calculation results showed that the DO influenced their normal growth and metabolism of NSCs mostly in the in vitro culture within collagen-based scaffolds. This study also confirmed that 2-mm thickness of collagen scaffold was capable of in vitro cultivation and growth of NSCs with an inoculating density of 1 x 10(6) cells/mL.