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论文类型：期刊论文

发表时间：2011-11-30

发表刊物：EUROPEAN JOURNAL OF PHARMACOLOGY

收录刊物：Scopus、SCIE、PubMed

卷号：670

期号：2-3

页面范围：471-478

ISSN号：0014-2999

关键字：Protocatechuic acid; Neural stem and progenitor cell; Differentiation; Neuron; Neuroprotection

摘要：Protocatechuic acid (PCA), a phenolic compound isolated from the kernels of Alpinia (A.) oxyphylla, plays crucial roles in the proliferation and neuroprotection of cultured neural stem and progenitor cells (NS/PCs) in our previous study. However, whether PCA modulates the differentiation of NS/PCs has remained to be elucidated. In this study, we show that PCA can promote the neuronal differentiation combined with fetal bovine serum (FBS) in vitro, although it cannot initiate the differentiation of NS/PCs by itself. Moreover, PCA is able to induce neuronal maturation and efficiently promote neurite outgrowth. On the other hand, PCA facilitates survival of phenotypes differentiated from cultured NS/PCs, which was associated with an increased percentage of the cellular viability and a decreased percentage of cells undergoing apoptosis under differentiation conditions. In addition, PCA-induced survival is also mediated with the activating of endogenous antioxidant enzymes. These results suggest that PCA may serve as a useful reference for future studies in designing stem cell strategies to promote brain recovery and repair in neurodegenerative diseases. (C) 2011 Elsevier B.V. All rights reserved.