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个人信息Personal Information
教授
博士生导师
硕士生导师
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:化工学院
学科:化学工程. 工程热物理
办公地点:化工学院 化工实验楼 D-309
联系方式:辽宁省大连市凌工路2号 大连理工大学化环生学部化工学院 116024
电子邮箱:xuehuma@dlut.edu.cn
Thermoresponsive Copolymer Nanofilms for Controlling Cell Adhesion, Growth, and Detachment
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论文类型:期刊论文
发表时间:2010-11-16
发表刊物:LANGMUIR
收录刊物:SCIE、EI、PubMed、Scopus
卷号:26
期号:22
页面范围:17304-17314
ISSN号:0743-7463
摘要:This study reports the development and use of a novel thermoresponsive polymeric nanofilm for controlling cell adhesion and growth at 37 degrees C, and then cell detachment for cell recovery by subsequent temperature drop to the ambient temperature, without enzymatic cleavage or mechanical scraping. A copolymer, poly(N-isopropylacrylamide-co-hydroxypropyl methacrylate-co-3-(trimethoxysilyl)propyl methacrylate) (abbreviated PNIPAAm copolymer). was synthesized by free radical polymerization. The thermoresponses of the copolymer in aqueous solution were demonstrated by dynamic light scattering (DLS) through detecting the sensitive changes of copolymer aggregation against temperature. The DES measurements revealed the lower critical solution temperature (LCST) at approximately 30 degrees C The PNIPAAm film stability and robustness was provided through silyl cross-linking within the film and with the hydroxyl groups on the substrate surface. Film thickness, stability, and reversibility with respect to temperature switches were examined by spectroscopic ellipsometry (SE), atomic force microscopy (A FM), and contact angle measurements. The results confirmed the high extent of thermosensitivity and structural restoration based on the alterations of film thickness and surface wettability. The effective control of adhesion, growth, and detachment of HeLa and HEK 293 cells demonstrated the physical controllability and cellular compatibility of the copolymer nanofilms, These PNIPAAm copolymer nanofilms could open up a convenient interfacial mediation for cell film production and cell expansion by nonenzymatic and nonmechanical cell recovery.