马学虎

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:男

毕业院校:大连理工大学

学位:博士

所在单位:化工学院

学科:化学工程. 工程热物理

办公地点:化工学院 化工实验楼 D-309

联系方式:辽宁省大连市凌工路2号 大连理工大学化环生学部化工学院 116024

电子邮箱:xuehuma@dlut.edu.cn

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Enhancement of Adipose-Derived Stem Cell Differentiation in Scaffolds with IGF-I Gene Impregnation Under Dynamic Microenvironment

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论文类型:期刊论文

发表时间:2010-10-01

发表刊物:STEM CELLS AND DEVELOPMENT

收录刊物:SCIE、PubMed、Scopus

卷号:19

期号:10

页面范围:1547-1556

ISSN号:1547-3287

摘要:Biochemical and mechanical signals enabling cardiac regeneration can be elucidated by using in vitro tissue engineering models. We hypothesized that human insulin-like growth factor-I (IGF-I) and 3-dimensional (3D) dynamic microenvironment could enhance the survival and differentiation of adipose tissue-derived stem cells (ADSCs). In this study, ADSCs were cultured on 3D porous scaffolds with or without plasmid DNA PIRES2-IGF-I in cardiac media, in static culture dishes, and in a spinning flask bioreactor, respectively. Cell viability, formation of cardiac-like structure, expression of functional proteins, and gene expressions were tested in the cultured constructs on day 14. The results showed that dynamic microenvironment enhanced the release of plasmid DNA; the ADSCs can be transfected by the released plasmid DNA PIRES2-IGF-I in scaffold. IGF-I showed beneficial effects on cellular viability and increase of total protein and also increased the expressions of cardiac-specific proteins and genes in the grafts. It was also demonstrated that dynamic stirring environment could promote the proliferation of ADSCs. Therefore, IGF-I, expressed by ADSCs transfected by DNA PIRES2-IGF-I incorporated into scaffold, and hydrodynamic microenvironment can independently and interactively increase cellular viability and interactively increase the expression of cardiac-specific proteins and genes in the grafts. The results would be useful for developing tissue-engineered grafts for myocardial repair.