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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：化学工程. 工程热物理
办公地点：化工学院 化工实验楼 D-309
联系方式：辽宁省大连市凌工路2号 大连理工大学化环生学部化工学院 116024
电子邮箱：xuehuma@dlut.edu.cn
Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition
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论文类型：期刊论文
发表时间：2009-07-01
发表刊物：BIOTECHNOLOGY LETTERS
收录刊物：SCIE、EI、PubMed
卷号：31
期号：7
页面范围：923-928
ISSN号：0141-5492
关键字：Hematopoietic stem cells; Hypoxia; Microcapsulation; Osteoblasts; Stem cells
摘要：The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34(+) phenotypic analysis with flow cytometry. At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34(+) cells had achieved 18.7 +/- A 1.6, 11.6 +/- A 0.9 and 23.4 +/- A 2-fold expansions, respectively, in the test groups. These were significantly different from those in control groups. Microencapsulated osteoblasts under hypoxia conditions had therefore a significant effect on the expansion potential of HSPCs in vitro.