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Indexed by:期刊论文

Date of Publication:2022-06-29

Journal:高等学校化学学报

Affiliation of Author(s):国际教育学院（留学生办公室）

Volume:39

Issue:09

Page Number:1913-1918

ISSN No.:0251-0790

Abstract:Superhydrophobic polystyrene film with low water sliding angle was prepared by electrospinning, and the microdroplet detection technology of protein based on the superhydrophobic film was established. The superhydrophobic surface ensures the formation of spherical droplets, and the amount of sample required was thus decreased from hundreds of microliters to 10 mu L. The low water sliding angle facilitates the manipulation of microdroplets and recycling of the detection substrates. Rapidly qualitative and sensitively quantitative detection of bovine serum albumin (BSA) molecules was achieved by the combination of biuret colorimetric assay and the microdroplet detection technology based on the superhydrophobic surface. Compared with the solution phase detection, the detection limit was decreased from 24.53 mu mol/L to 1.490 mu mol/L after drying the microdroplet on the electrospining polystyrene (PS) film due to the better concentration effect of the analytes on the superhydrophobic surface. This microdroplet detection technology of protein has a certain degree of universality. This study provides a new technical platform for the highly sensitive detection of trace biological/chemical molecules.

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