戴建英   

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Language:English

Paper Publications

Title of Paper:发酵条件对产2,3-T二醇Klebsiella pneumoniae代谢的影响

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Date of Publication:2010-01-01

Journal:食品与发酵工业

Included Journals:PKU、ISTIC、CSCD

Volume:36

Issue:8

Page Number:6-11

ISSN No.:0253-990X

Key Words:2,3-丁二醇; 发酵; 代谢特性

Abstract:通过批式发酵实验考察了通气量和pH对产2,3-丁二醇的Klebsiella pneumonine CICC
   10011代谢特性的影响,确定了发酵2,3-丁二醇的最适条件为:通气量0.10
   vvm,pH值5.8.在该条件下发酵8h,目标产物浓度达到15.76 g/L,产物得率为0.32 g/g葡萄糖,生产强度为1.97
   g/(L.h).对发酵过程中细胞生长和主要代谢产物的形成进行分析,结果表明,早期乙酸的形成有利于目标产物2,3-丁二醇的积累,后期有机酸用作底物
   维持细胞生存.调节底物浓度、通气量和pH,使细胞尽早产生足够的乙酸,维持较长的对数生长期是发酵生产2,3-丁二醇的关键

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